Figure 6: Selection thresholds based on a prototypic ANA H-CDR3.

(a,b) Flow cytometry analysis of spleen cells. (a) Plots show surface expression of CD80 and PD-L2 on B cells from control and SLC^−/− mice. Plots show CD73 expression on CD80⁺PD-L2⁺ and CD80⁻PD-L2⁻ B cells from SLC^−/− mice. (b) Histogram shows IgM expression on CD80⁺PD-L2⁺CD73⁺ B cells, bar indicates % IgM⁻ cells. (c) Analysis of expressed V_HDJ sequences in sorted naive, GC and memory B cells. Pie charts show the proportions of V_H sequences that carry 1, 2, 3 and so on point mutations in indicated cell populations. Below is shown the average number of mutations per V_H. (d,e) Proportions of H-CDR3 sequences with 0, 1 or ≥2 arginine (R) and/or lysine (K) residues in indicated cell populations. (f,g) H-CDR3 length distribution in indicated cell populations, with unique and identical sequences, pink and black bars, respectively. Data are representative from at least two independent experiments (a,b). Data are from a pool of six (c,d,f) and five (e,g) SLC^−/− mice. The number of sequences analysed are at the pie chart centre.