Figure 4: RNA Pol II during CSR in 3′RR-deficient mice.
From: Elucidation of IgH 3′ region regulatory role during class switch recombination via germline deletion
ChIP assays were performed with splenic B cells from 3′RR-deficient and wt mice. Cells were stimulated with LPS+IL-4 for 2 days. RNA Pol II P-ser 2 (a). RNA Pol II P-ser 5 (b). Background signals from mock samples with irrelevant antibody were subtracted. Values were normalized to the total input DNA. Data are the mean±s.e.m. of three experiments with two mice for each genotype. *P<0.05 and **P<0.01 (Mann–Whitney U-test).
