Figure 1: IEL supernatant (SN) induces antiviral defence pathways in IEC.
From: Intestinal intraepithelial lymphocyte activation promotes innate antiviral resistance
(a) Hierarchical clustering of differentially expressed genes in MODE-K cells treated with SN from unstimulated cultured IEL and SN from IEL activated on plate-bound anti-CD3 for 24 h, in comparison to genes expressed in MODE-K cultured in medium alone. Heatmap key: red=higher expression, blue=lower expression relative to the mean expression of the gene across all samples. (b) Genes upregulated in MODE-K by stimulated IEL SN relative to unstimulated IEL SN were subjected to enrichment analysis using Metacore. Shown are the top ten statistically significant pathways, calculated using an adjusted P value of 0.05. (c) Confirmation by qRT–PCR of upregulation of some antiviral genes. MODE-K cells were cultured for 18 h with medium, anti-CD3, or SN from cultured IEL as in a. Data are the mean and s.e.m. of three to five independent experiments calculated as fold change relative to the untreated samples. See also Supplementary Fig. 1.
