Figure 4: MiR-125a−/− mice are susceptible to autoimmune EAE.
From: MiR-125a targets effector programs to stabilize Treg-mediated immune homeostasis
(a)Mean clinical scores (±s.e.m.) of EAE induced in 6- to 8-week-old WT and miR-125a-deficient mice. (b) Representative haematoxylin–eosin (H&E) staining or Luxol Fast Blue staining of spinal cord sections on day 21 after immunization of the mice in a. (c) Quantitative RT–PCR analysis of gene expression in splenic CD4+ T-cell samples on day 21 after immunization of the mice in a. The relative expression levels of different genes were normalized to the expression of mouse Rpl13a. (d) Enzyme-linked immunosorbent assay examination of the concentration of IFN-γ and IL-17a in the supernatants from the splenocyte culture challenged with MOG peptide. (e,g) Representative flow cytometric analysis of the percentage of Treg, TH1 and TH17 cells in the CD4+ T cells isolated from the spleens (e) and CNS (g) of the mice (a) on day 21 after immunization. (f,h) The percentages of Foxp3+ Treg cells, IFN-γ+ T cells and IL-17+ T cells in the CD4+ populations from the spleens (e) and CNS (g), respectively. *P<0.05, **P<0.01 versus control (two-tailed Student’s t-test). Data are representative of three (a,b, e–h) or four (c,d) independent experiments (mean±s.e.m.).
