Figure 6: IFNγ from endogenous CD8 T cells controls CCL21 but not PNAd expression in i.p. tumours.
Infiltration of naive OT-I cells into i.p. (a,b,d) or s.c. (c) B16-OVA tumours in the indicated mice was determined as in Fig. 2. n=7 (a), 3 (b,d) and 5 (c). In d, Rag2−/− mice were repleted with either WT or IFNγ−/− CD8 T cells before tumour implantation. (e) The absolute number of CD8+ T cells (non-OT-I) that had accumulated in i.p. B16-OVA tumours in mice with the indicated background was determined by flow cytometry. n=3 per group. (f,g) PNAd expression in i.p. tumours from the indicated mice was quantified as in Fig. 3. n=3 per group. Ccl21 (h,j) or Ccl19 (i) expression in i.p. tumours lysates from the indicated mice was quantified. Ccl21 data (h,j) are presented as 2^−ΔΔCT method relative to HPRT with expression levels in control samples normalized to 1. Ccl19 data (i) is presented as 2^−ΔCT relative to HPRT. Dashed line represents Ccl21 expression levels in control tumours. n=3–6 per group. Data (mean+s.e.m.) are representative at least two independent experiments. ns: P>0.05, *P<0.05, **P<0.01, ***P<0.001 by unpaired t-test (a–c,f–i) or one-way analysis of variance with Tukey’s post test (d,j).
