Figure 2: Super-resolution imaging with Oligopaints and STORM.

(a) Schematic illustrating how a diffraction-limited FISH signal presents as many smaller fluorescence localizations via STORM. (b) Simulated STORM images of two polymer models (left) illustrating the importance of localization density in resolving structure (total localizations in upper right corners). The colour code on the polymer models traces along the length of the polymer (black to red to white). (c) Average number of localizations (mean±s.e.m.; n=434 for unlabeled/Cy5, n=133 for Cy5/Cy5, n=353 A405/Cy5) per BX-C locus in Drosophila clone 8 cells when the unlabelled primary probe is paired with a secondary oligo carrying Cy5 (left), when both the primary probe and secondary oligo carry Cy5 (middle), and when the primary probe carrying an AlexaFluor 405 activator is paired with a secondary oligo carrying Cy5. (d) Conventional (left) and STORM (right) images of the BX-C locus from three cells, with cell shown in bottom row exhibiting two loop-like protrusions. The conventional and STORM images depict the same field of view at the same magnification. Right two panels: zoomed-in views of the boxed regions. (e) Simulation in which two-thirds of the localizations shown in image (d) have been removed at random to illustrate the loss of connectivity and structure in regions represented by a low density of localizations. (f) Conventional (left) and STORM (middle and right) images of a 5-kb region at 89B from three cells. Right panel: zoomed-in views of the centre panels. (g) A graph of the normalized number of photons detected (Normalized counts) per position (nm) in the x axis (dashed line) of the field shown in the bottom-right panel of f. The FWHM of the brightest feature is presented above the graph. Super-resolution images are presented as heat maps of single-mole localization density: black (fewest) -> red -> yellow -> white (most).