Figure 5: miR-21 deletion protects mice from TNF-α-induced shock.

(a) Survival of WT and miR-21^−/− mice challenged with 9 μg TNF-α (n=10 per group, P<0.0001). (b) Serum samples of WT and miR-21^−/− mice were collected 3 h after challenge with 9 μg TNF-α and LDH activities were analysed (n=5 per group, a separate cohort). Representative histological sections of the ileum (c), kidney (e) and liver (g) stained with haematoxylin and eosin 3 h after TNF-α injection in WT and miR-21^−/− mice. Scale bars, 200 μm for the ileum and 100 μm for the kidney and liver. (n=5 for each group). The tissue damage score for the ileum (d), kidney (f) and liver (h) was evaluated. *P<0.05 and **P<0.01 indicate a significant difference between WT and miR-21^−/− mice treated with TNF-α (two-way analysis of variance). NS, not significant.