Figure 4: Global or myeloid-specific IL-4Rα deficiency is protective against CP.
From: Alternatively activated macrophages promote pancreatic fibrosis in chronic pancreatitis
WT, IL-4/IL-13−/−, IL-4Rα−/− and LysMcreIL-4Rαflox/flox mice were subjected to caerulein-induced CP. (a) Relative pancreas weight (pancreas weight/body weight) are shown. n=6–7 per genotype, means±s.e.m., ***P<0.001, ns, not significant, P<0.05 considered significant (one-way analysis of variance (ANOVA), Tukey’s post hoc test). (b) Histologic feature of the pancreas from different genotypes of mice are shown using H&E and Trichrome staining. Scale bar, 200 μm. (c,d) Quantitative RT–PCR analysis of fibrosis-associated genes αSMA (α-SMA) and Col1α1 (Collagen1A1) in the pancreas of indicated mice is shown. Means±s.e.m. *P<0.05, **P<0.01, ns, not significant (one-way ANOVA, Tukey’s post hoc test). (e) Representative immunofluorescence images of the pancreas from each genotype of mice are shown (co-stained with α-SMA, Collagen1A1 and DAPI). (f) Flow cytometry analysis of pancreatic macrophages (CD11b+F4/80+) CD206 expression (MFI) from different genotypes of mice is shown. Means±s.e.m., *P<0.05, **P<0.01, ns, not significant (one-way ANOVA, Tukey’s post hoc test).
