Figure 5: IL-4/IL-13-blocking peptide ameliorates established CP.
From: Alternatively activated macrophages promote pancreatic fibrosis in chronic pancreatitis
IL-4/IL-13-blocking peptide (IL4/13BP i.p., 50 μg per mouse ,5 days per week) was administrated to mice 2 weeks after starting CP induction and mice were killed as previous after 4 weeks of caerulein injections. (a) Relative pancreas weight from Con and IL4/13BP-treated mice are shown. n=8–9 per group, means±s.e.m. (unpaired two-tailed Student’s t-test). (b) Representative of pancreas H&E and Trichrome staining. Scale bar, 200 μm. (c) Representative immunofluorescence images of the pancreas from indicated mice (co-stained with α-SMA, Collagen1A1 and DAPI). Scale bar: 50 μm. (d,e) RT–PCR analysis of α-SMA and Col1α1 (Collagen1A1) gene expression in the pancreas of indicated mice. Means±s.e.m. (unpaired two-tailed Student’s t-test) (f) Flow cytometry analysis of CD206 expression (MFI) by pancreatic macrophages (CD11b+F4/80+) isolated from indicated mice. Means±s.e.m. (unpaired two-tailed Student’s t-test). (g) Human macrophages as above were cultured with control medium (Con), conditioned medium from hPSCs (hPSC_CM) or hPSC_CM pretreated with 1 μM IL4/13BP (hPSC_CM+BP) for 24 h. Expression of CD206 (% CD206+ macrophages) was analysed using flow cytometry.
