Figure 4: Exosome secretion promotes cell migration.
From: Directional cell movement through tissues is controlled by exosome secretion
(a) Average number of exosomes secreted from endolysosomal secretion-inhibited HT1080 cell lines from n≥3 experiments. (b) Western blots of exosome cargos. FLOT, flotillin. (c) Migration speed of Rab27a-knockdown (KD) HT1080 cells in exosome-depleted media±add-back of purified exosomes (Exo), as indicated on tissue culture-treated plates. (d) Speed of Rab27a-KD cells on exosome-coated plates. (e) Persistence index calculated from experiments shown in d. (f) Wind–Rose plots for data shown in d, Exo 0. Magnification of boxed area is shown at the right. (g) Western blot for FN-depleted exosomes. Numbers below blot represent relative intensity (Int.) of FN (normalized to FLOT) from that blot. WCL, whole-cell lysates; (h) Single-cell migration assays on FN-depleted exosome-coated plates. Error bars, s.e.m., from n≥29 cells for each cell line from three independent experiments. *P<0.05; **P<0.01; ***P<0.001 compared with Sc by Student t-test. #P<0.05; ##P<0.01; ###P<0.001 compared with same cell line on Exo 0 by Student’s t-test. NS, not significant.
