Figure 3: Stk38 inhibits TLR9-mediated ERK1/2 activation.

(a,b) Peritoneal macrophages from wild-type and Stk38-deficient mice were stimulated with 10 μg ml⁻¹CpG (a) or 100 ng ml⁻¹ LPS (b) for indicated time. Phosphorylated and total ERK1/2, p38, JNK and NF-κB p65 were detected by western blot. Similar results were obtained in three independent experiments. (c) HEK 293T cells were transfected with 50 ng MyD88-expressing plasmid, 100 ng Elk1 luciferase reporter plasmid, 2.5 ng pTK-Renilla-luciferase, together with 0, 100 or 200 ng of Stk38-expressing plasmid. Total amounts of plasmid DNA were equalized using control (Ctrl) empty vector. After 24 h of culture, luciferase activity was measured and normalized by Renilla luciferase activity. Data are shown as mean ±s.d. (n=4) of one typical experiment (**P<0.01, Student’s t-test). Similar results were obtained in three independent experiments.