Figure 4: Channel properties of the C1C2GA mutant in HEK cells and mouse neurons.
From: Atomistic design of microbial opsin-based blue-shifted optogenetics tools
(a) Photocurrents of C1C2WT and its GA mutant. Photocurrents in C1C2-expressing HEK293 cells were measured at 16 different holding potentials. (b) The peak amplitudes of the photocurrents, normalized by the cell’s input capacitance. Values are means and s.e.m. of 8–10 experiments. (c,d) The peak amplitudes (c) and photocurrents (d) of the C1C2GA mutant in mouse neurons. (e) Current-clamp mode recording of C1C2GA, using light pulses at different wavelengths (100 ms, 1–2 Hz).
