Figure 1: eIF1A interacts with Ago2.
From: eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference
(a) Proteins co-immunoprecipitated with Flag-HA-Ago2 from HEK293 cells were separated by SDS–polyacrylamide gel electrophoresis (silver staining) and analysed by mass spectrometry. eIF1A is a novel Ago2-interacting protein (* indicates the detected peptides of eIF1A). (b) Fluorescence immunostaining was performed with anti-eIF1A (monoclonal) and anti-Ago2 (monoclonal) antibodies and DAPI (4′,6-diamidino-2-phenylindole) in HEK293 and human glioblastoma multiforme T98G cells. White arrows show the co-localized eIF1A and Ago2. (c) eIF1A interacts with Ago2. Human eIF1A was transiently overexpressed in stable HEK293 cells stably expressing Agos (1∼4). Immunoprecipitation assays were performed with anti-eIF1A (monoclonal) antibody and immunoblotting (IB) with anti-Agos antibodies in the upper panel and anti-eIF1A antibodies in the lower panel.
