Figure 2: Characteristics of genes under translational regulation.
From: Translational regulation shapes the molecular landscape of complex disease phenotypes
(a) Black slopes approximated 1 (x=y) and indicated equal fold changes of expression differences between SHR/Ola and BN-Lx across both levels of regulation for RNA+RIBO genes (standardized major-axis estimation; SMA). Significant (P<2.2e−16) divergence of blue and red slopes demonstrated translational regulation for RNAonly and RIBOonly genes, respectively. (b) Strain-specific differences of translationally regulated RIBOonly (n=179) genes were confirmed in the liver proteome (Wilcoxon-Mann-Whitney; P<1e−4). Variation in translation captured by Ribo-seq resulted in significantly different protein abundances across strains (whiskers indicate 5th and 95th percentiles). RNAonly genes have a lower (c) translational efficiency and a significantly increased (d) 3′UTR length compared with RIBOonly and RNA+RIBO genes (mean with 95% confidence interval).
