Figure 1: Technical replication of MCC-Seq methylation calls and comparison with WGBS. | Nature Communications

Figure 1: Technical replication of MCC-Seq methylation calls and comparison with WGBS.

From: Characterization of functional methylomes by next-generation capture sequencing identifies novel disease-associated variants

Figure 1

Correlation between technical replicates from a DNA sample derived from VAT sequenced from independent captures (a) of the same MCC-Seq sequence panel (Met V1) (4-plex and 10-plex; N=1,587,026 CpGs; R=0.98) and (b) of two different MCC-Seq sequence panels (Met V14-plex and Met V26-plex; N=1,569,170 CpGs; R=0.97). (c) Comparison between MCC-Seq4-plex and WGBS (N=1,620,874 CpGs; R=0.97) methylation calls for the same VAT DNA sample. Only CpGs with sequence coverage ≥5X in MCC-Seq and WGBS experiments were included in the analysis; R is the Pearson's correlation coefficient.

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