Figure 1: Total chemical synthesis and functional validation of wild-type and activity-caged hCCL5. | Nature Communications

Figure 1: Total chemical synthesis and functional validation of wild-type and activity-caged hCCL5.

From: Chemical synthesis of a two-photon-activatable chemokine and photon-guided lymphocyte migration in vivo

Figure 1

(a) General strategy for chemical synthesis of hCCL5 and its photo-caged forms. (b) Transwell migration assay for wild-type hCCL5 and DMNB-caged versions before and after irradiation with ultraviolet light (365 nm) for 5 min. (c) HPLC traces for one-pot synthesis of hCCL5**. (d) Chemical structure of NDBF-modified Ser residue. (e) Transwell migration assay for two-photon caged hCCL5** before and after exposure to ultraviolet light (365 nm) for 30 s. Data representative of three independent experiments are shown both in b and e. Error bars indicate s.e.m. The image in a is modified from Protein Data Bank file 1RTO.

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