Figure 7: Formation of circular TatBC oligomers through specific contact sites of TatC.
From: Initial assembly steps of a translocase for folded proteins
(a) Model of a TatBC tetramer looking down the membrane normal from the trans-side of the Tat translocase. Residues D63 and A133 of each TatC monomer are highlighted. (b) Membrane vesicles (INV) containing TatAB and either a Cys-less mutant of TatC (TatCΔCys) or the indicated single and double Cys mutants of TatC were either incubated with the sulfhydryl specific homobifunctional crosslinker BMH or mock treated. Proteins were dissolved in DTT-free SDS–PAGE loading buffer and analysed for the formation of TatC oligomers (blue stars) by SDS–PAGE and western blotting using anti-TatC antibodies.
