Figure 4: Absence of the post-commissural fornix in E18.5 MAP6-KO embryos. | Nature Communications

Figure 4: Absence of the post-commissural fornix in E18.5 MAP6-KO embryos.

From: Microtubule-associated protein 6 mediates neuronal connectivity through Semaphorin 3E-dependent signalling for axonal growth

Figure 4

(a) Sagittal sections with a cutting angle of 30° of E18.5 WT and KO brain were double immunostained with anti L1 CAM and Neuropilin1 (Nrp1). A diagram shows the projection stack of post-commissural fornix in red on a coronal plan. The green line indicates the plane of the sagittal sections. Note that the size of the anterior commissure is strongly reduced and the post-commissural fornix is absent. Scale bars, 300 μm. (b–e) Coronal section of E18.5 WT and MAP6-KO brain after anterograde DiI tracing of the fornix from the subiculum. For each panel, a sagittal diagram shows the projection stack of post-commissural fornix in red and a grey line indicates the level of coronal plan. Dashed square indicates the position of microscopic field. Scale bars, b, c 100 μm; d,e 50 μm. ac=anterior commissure; cc=corpus callosum; cf=column of the fornix, fi=fimbria; LV=lateral ventricle; pf=post-commissural fornix; Spt=septum; V3=third ventricle.

Back to article page