Figure 1: Comparison of SIM and epifluorescent imaging of platelet actin nodules. | Nature Communications

Figure 1: Comparison of SIM and epifluorescent imaging of platelet actin nodules.

From: Platelet actin nodules are podosome-like structures dependent on Wiskott–Aldrich syndrome protein and ARP2/3 complex

Figure 1

Epi-fluorescent images (left hand panel) of (a) human and (b) mouse platelets spreading on fibrinogen and stained for F-actin with Alexa488-phalloidin. The actin nodules in the boxed regions are shown enlarged in the zoomed image. The right hand panel shows SIM images of the same platelets with increased detail seen in the boxed actin nodule shown in the zoomed picture. Arrows indicate the actin fibres radiating from and connecting different actin nodules together. (c) Scanning electron micrograph of a representative human platelet displaying actin nodules. The right panel shows an enlargement of the boxed region in the left panel. Regions that could be multiple bright foci of actin are indicated by the arrowheads and interconnecting fibres by the arrows. Scale bars, 2 μm main image, boxed region: 0.5 μm.

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