Figure 2: Organization of integrin at actin nodules using SIM. | Nature Communications

Figure 2: Organization of integrin at actin nodules using SIM.

From: Platelet actin nodules are podosome-like structures dependent on Wiskott–Aldrich syndrome protein and ARP2/3 complex

Figure 2

(a) Z-projections of a 3D SIM image of a human platelet stained for F-actin with Alexa568-phalloidin (left panel), FITC-anti-αIIb (middle panel) and the merged image (right panel, actin=magenta, integrin=green). Arrowheads in middle panel indicate integrin depleted zones corresponding with the position of actin nodules. (b) Line scans showing the intensity of actin (magenta) and integrin (green) fluorescence signal at an area where there is no actin nodule (dotted line), at an actin nodule showing integrin depleted region (dashed line) and at an actin nodule showing integrin depleted region and edge enrichment (solid line). Scale bars, 2 μm.

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