Figure 4: Organization of talin and vinculin at actin nodules using SIM. | Nature Communications

Figure 4: Organization of talin and vinculin at actin nodules using SIM.

From: Platelet actin nodules are podosome-like structures dependent on Wiskott–Aldrich syndrome protein and ARP2/3 complex

Figure 4

SIM images of human platelets stained with Alexa488-phalloidin (left panel) and (a) Alexa568-anti-vinculin (middle panel) or (b) Alexa568-anti-talin. The merged images are shown in the right hand panel (actin=magenta, vinculin/talin=green) showing localization of vinculin and talin at actin nodules. In (a) and (b), arrows indicate the actin-rich nodule core and arrows head the ring of vinculin or talin around this core. (c) Quantification of the pattern of localization of talin and vinculin at the actin nodules by line scan intensity profiles. The line scan profile was scored as either enriched around the nodule; enriched at the centre of the actin nodule core; or no obvious enrichment at the nodule and the percentage of nodules displaying each pattern was plotted. Bars represent mean±s.d. from three independent experiments. In total 738 nodules were measured for talin and 667 nodules were measured for vinculin. Scale bars, 2 μm.

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