Figure 7: WASp is required for the formation of actin nodules in mouse and human platelets. | Nature Communications

Figure 7: WASp is required for the formation of actin nodules in mouse and human platelets.

From: Platelet actin nodules are podosome-like structures dependent on Wiskott–Aldrich syndrome protein and ARP2/3 complex

Figure 7

(a) SIM images of Alexa488-phalloidin labelled platelets from wild type (left panel), WASp KO (middle panel) and WASp Y293F (right panel) mouse platelets. Platelets from both the WASp KO and WASp Y293F did not form conventional actin nodules as seen in wild-type platelets. (b) Quantification of the number of platelets displaying actin nodules. Data are means±s.d. (n=3). ***P<0.001 from one way ANOVA. (c) Quantification of the surface area of spread platelets from wild type, WASp-KO and WASp-Y293F mice. Data are means±s.d. (n=3), *P<0.05 from one way ANOVA. Approximately 400 platelets were analysed for each genotype. (d) SIM images of Alexa488-phalloidin labelled human platelets from control (left panel) and Wiskott–Aldrich syndrome patient (WAS patient#2—right panel). Platelets from the patient samples did not form actin nodules as could be seen in the control samples. (e) Quantification of the percentage of platelets per field of view displaying actin nodules. Data are means±s.d. (f) Quantification of the surface area of spread platelets from control and WAS patients. Data are means±s.d. *P=0.02 from ANOVA and Tukey’s multiple comparisons. More than 125 platelets were analysed for each control and patient sample. Scale bars, 2 μm.

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