Figure 8: Comparison of Arp2/3 localization in control and WAS patient platelets. | Nature Communications

Figure 8: Comparison of Arp2/3 localization in control and WAS patient platelets.

From: Platelet actin nodules are podosome-like structures dependent on Wiskott–Aldrich syndrome protein and ARP2/3 complex

Figure 8

(a) SIM images of human platelets stained with Alexa488-phalloidin (left panel) and Alexa568-anti-p34 (middle panel) and the merged image (right panel, actin=magenta, p34=green). The localization of ARP2/3 complex at actin nodules can be observed in control platelets (top row, arrows indicate two examples); however, in WASp platelets no actin nodules and therefore no bright foci of ARP2/3 complex were observed. (b) The localization of ARP2/3 complex at lamellipodia in fully spread platelets is the same in both control and WAS patient samples indicating that ARP2/3 complex activity downstream of other NPFs is unaffected by the loss of WASp. Scale bars, 2 μm.

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