Figure 9: Effect of WASp KO on platelet adhesion and aggregation under flow conditions. | Nature Communications

Figure 9: Effect of WASp KO on platelet adhesion and aggregation under flow conditions.

From: Platelet actin nodules are podosome-like structures dependent on Wiskott–Aldrich syndrome protein and ARP2/3 complex

Figure 9

Differential interference contrast (DIC) microscopy images of a representative field of view (FOV) from (a) WT and (b) WASp-KO mouse blood flowed over collagen-coated capillaries at a shear rate of 3,000 s−1 (left panel) with an enlargement of the boxed region (right panel). The failure of WASp-KO platelets to form aggregates can clearly be seen. Quantification of the aggregates in terms of their (c) size and (d) volume. DIC images of (e) WT and (f) WASp-KO mouse blood flowed over fibrinogen coated capillaries at a shear rate of 600 s−1 (left panel) with an enlargement of the boxed region (right panel). The reduction in both the number of platelets adhered and the extent of their spreading is clearly observed and is quantified in (g) and (h). Scale bars, 10 μm. Bars in (c,d,g and h) represent mean±s.e.m. of 10 FOV per experiment from three independent experiments.

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