Figure 7: PfCDPK1 phosphorylated at serine-64 is associated with apical parasite structures. | Nature Communications

Figure 7: PfCDPK1 phosphorylated at serine-64 is associated with apical parasite structures.

From: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion

Figure 7

(a) A schizont stage parasite stained with DAPI to reveal the nuclei (blue), an antibody to PfEBA-175 to reveal the micronemes (red) and the phospho-specific CDPK1-pS64 antibody (green). A merge of images from all three stains is shown on the far right; this is a representative image from at least three experiments. Also shown is a rendered image where deconvoluted z stacks (see: Supplementary Movie 3) were reconstructed in 3D, with interpolation. The inset shows a rendered image of a free merozoite from the same preparation (see: Supplementary Movie 4). (b) The same as a, but instead of probing with an anti-EBA-175 antibody the preparation was probed with an antibody to the rhoptry marker PfTRAMP (red;see: Supplementary Movie 5 (schizonts) and Supplementary Movie 6 (merozoites) for z stacks). Scale bars, a=1 μm (insert=0.5 μm), b=1 μm (insert=0.5 μm),

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