Figure 9: Parasite red blood cell invasion is dependent on PfPKG activity.

(a) Invasion of wild-type free merozoites into red blood cells was monitored in the absence or presence of Compound 2 (2 μM; Comp 2) by the determination of the number of infected red blood cells 24 h post invasion. Comp 2 was also added 1 h post infection and the number of infected red blood cells 24 h later was determined (Comp 2 Control); this was to control for the possibility that Comp 2 treatment might affect the survival of the parasites post invasion. (b) The invasion of PfPKG_T618Q merozoites into red blood cells was monitored. The results are the mean of 3–5 experiments ±s.e.m. Student’s paired t-test was applied to test statistical significance. ***P<0.001, *P<0.05.