Figure 2: SENP2 can process SUMOylation induced after heat shock under denaturing conditions.
From: System-wide identification of wild-type SUMO-2 conjugation sites
(a) HeLa cells were either incubated at 43 °C for 1 h or mock treated. Subsequently, the cells were lysed in 8 M urea, homogenized and subsequently diluted to reduce the concentration of urea as indicated.. Lysates were treated with SENP2 or mock treated. After protease treatment, lysates were size-separated by SDS–PAGE, transferred to membranes, probed using a SUMO-2/3 antibody and visualized using chemiluminescence. The experiment was performed in biological duplicate. (b) As in a, but with total protein content visualized using Ponceau-S staining.
