Figure 1: PriX is a novel noncatalytic subunit of the primase from S. solfataricus.
From: A primase subunit essential for efficient primer synthesis by an archaeal eukaryotic-type primase
(a) Co-immunoprecipitation of the S. solfataricus cell extract with anti-PriL antibodies. The cell extract was treated with DNase I/RNase A and subjected to immunoprecipitation with anti-PriL antibodies. C, no cell extract; IP, the immunoprecipitate; M, molecular weight markers; —, no anti-PriL antibodies. (b) Primer synthesis by PriS, PriL and PriX separately or in various combinations on M13 ssDNA with rNTPs as the substrates. PriX, PriS and/or PriL were incubated with M13 ssDNA in the standard assay mixture. A diagram depicting the template and the products (in red) is shown on top. (c) A time course of primer synthesis by PriSL, PriSX and PriSLX. PriSL, PriSX and PriSLX were incubated for indicated periods of time with dNTPs as the substrates on M13 ssDNA in the standard assay mixture. The relative activity corresponding to primer synthesis by PriSLX in a 20-min reaction is defined as 100%. Error bars represent s.d. of experimental triplicates.
