Figure 3: Free SA and SA glycoside levels in Arabidopsis thaliana F1 hybrids and their parents. | Nature Communications

Figure 3: Free SA and SA glycoside levels in Arabidopsis thaliana F1 hybrids and their parents.

From: Salicylic acid biosynthesis is enhanced and contributes to increased biotrophic pathogen resistance in Arabidopsis hybrids

Figure 3

(a) qPCR analyses of ICS1 and PR1 expression in F1 hybrids and parents of Col-0 × Sei-0 every 8 h post infiltration (hpi) up to 48 hpi with Pseudomonas syringae pv. tomato (Pst) DC3000 at 1 × 105 c.f.u. ml−1 and MgCl2. Fsc and Fcs, reciprocal F1 hybrids, where maternal line is Sei-0 and Col-0, respectively. (b) qPCR analyses of ICS1 and PR1 expression in F1 hybrids and parents of Col-0 × Aa-0 1 every 8 h post infiltration (hpi) up to 48 hpi with Pst DC3000 at 1 × 105 c.f.u. ml−1. Fac and Fca, reciprocal F1 hybrids, where maternal line is Aa-0 and Col-0, respectively. Data are standardised for abundance of Actin transcript. (c) Free SA and SAG levels in F1 hybrids and parents of two crosses 1 dpi with Pst DC3000 at 1 × 105 c.f.u. ml−1. The results are a representative of three biological repetitions. Error bars indicate s.d.. Data are shown as mean±s.d. (n=3, n means technical replication). **P<0.01 between hybrids and parents (Student’s t-test).

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