Table 1 Kinetic parameters of HP1α - chromatin interaction dynamics.
From: Multivalency governs HP1α association dynamics with the silent chromatin state
Experimental system | Dissociation kinetics | Association kinetics | Equilibrium | Replicates | |||||
|---|---|---|---|---|---|---|---|---|---|
Chromatin | Effector | τ off,1 (s) | % A1 | τ off,2 (s) | % A2 | λ on (s) | k on (M-1 s-1) × 10 6 | Kd (μM) * | n (n ′) † |
H3K9me3 (100%) | HP1α | 0.25±0.03 | 87±7 | 2.26±1.22 | 13±7 | 22.9±9.8 | 3.64±1.56 | 1.16±0.54 | 16 (8) |
H3K9me0 | HP1α | 0.08±0.03 | 83±10 | (3.70±4.90)‡ | 17±10 | N/A§ | N/A§ | N/A§ | 3 (2) |
H3K9me3 (75%) | HP1α | 0.19±0.01 | 95±3 | 4.03±4.09 | 5±3 | 51.0±25.8 | 1.63±0.82 | 3.23±1.63 | 5 (3) |
H3K9me3 (50%) | HP1α | 0.17±0.03 | 94±6 | 4.09±3.62 | 6±6 | 48.2±15.2 | 1.73±0.55 | 3.40±1.24 | 6 (4) |
H3K9me3 (25%) | HP1α | 0.15±0.02 | 95±2 | 4.57±3.13 | 5±2 | 75.8±23.0 | 1.10±0.33 | 6.06±1.99 | 6 (3) |
H3K9me3 (MN) | HP1α | 0.13±0.01 | 78±11 | (1.18±0.62)‡ | 22±11 | N/A§ | N/A§ | N/A§ | 3 (3) |
H3K9me3 (100%) | HP1α+50 nM comp. | 0.19±0.05 | 94±3 | 3.97±1.31 | 6±3 | 23.8±2.84 | 3.55±0.42 | 1.48±0.43 | 5 (3) |
H3K9me3 (100%) | HP1α+250 nM comp. | 0.13±0.02 | 93±2 | 3.93±1.73 | 7±2 | 43.3±15.8 | 2.07±0.62 | 3.72±1.25 | 3 (2) |
H3K9me3 (100%) | HP1α+1000, nM comp. | 0.11±0.01 | 93±4 | 2.75±0.72 | 7±4 | 29.3±5.51 | 2.92±0.57 | 3.11±0.67 | 4 (2) |
H3K9me3 (100%) | HP1αcdm | 0.33±0.01 | 87±1 | 3.40±0.53 | 13±1 | 7.45±1.87 | 22.4±0.6|| | 0.13±0.01 | 4 (2) |
H3K9me3 (100%) | HP1α (I163E) | 0.23±0.10 | 85±7 | (4.11±2.83)‡ | 15±7 | N/A§ | N/A§ | N/A§ | 4 (2) |
H3K9me3 (100%) | HP1α (W40A) | 0.10±0.02 | 85±21 | (0.56±0.49)‡ | 15±21 | N/A§ | N/A§ | N/A§ | 2 (2) |
H3K9me3 (100%) | HP1α+P3 | 0.33±0.04 | 95±4 | 4.80±3.96 | 5±4 | 10.5±0.59 | 7.94±0.45 | 0.38±0.05 | 4 (2) |
H3K9me3 (100%) | HP1α+P4 | 0.28±0.01 | 93±4 | 4.45±2.10 | 7±4 | 19.6±6.52 | 4.25±1.41 | 0.84±0.28 | 4 (3) |
