Figure 1: MiR-27b is involved in the drug resistance and tumorigenicity of breast cancer cells.
From: Loss of microRNA-27b contributes to breast cancer stem cell generation by activating ENPP1
(a) Relative miR-27b expression levels in normal and luminal-type breast cancer tissues. Expression levels were normalized to those of RNU6B. Differences between groups were analysed using unpaired t-tests. (b) Schematic illustration of the miR-27b sensor construct used in the experiments shown in c–f. (c) Expression of Zs-DR 48 h after transfection of MCF7-luc Zs-DR-27bs cells with a negative control or miR-27b-specific LNA. Scale bar, 100 μm. (d) Flow cytometric analyses of Zs-DR expression 48 h after treatment of MCF7-luc Zs-DR-27bs cells with dimethylsulphoxide (DMSO) or docetaxel (DOC). (e) Isolation of tumourigenic MCF7-luc Zs-DR-27bs cells. After transplantation of MCF7-luc Zs-DR-27bs cells into 5-week-old NOD/SCID mice, the cells were isolated and cultivated in vitro. (f) Flow cytometric analyses of Zs-DR expression in tumourigenic MCF7-luc Zs-DR-27bs cells isolated from the mice described in e.
