Figure 2: MiR-27b regulates the resistance of breast cancer cells to docetaxel.

(a) Overview of the method used to establish miR-27b knockdown MCF7-luc (MCF7-luc anti-miR-27b) cells. (b,c) Dose–response curves of MCF7-luc anti-NC, MCF7-luc anti-miR-27b and MCF7-luc miR-27b o.e. cells treated with docetaxel. Cell viability was normalized to that of the corresponding cells treated with dimethylsulphoxide (DMSO). The red dashed line indicates the IC₅₀ value. Data are represented as the mean±s.d. of n=3 replicates. (d) Morphologies of the MCF7-luc anti-NC, MCF7-luc miR-27b o.e. and MCF7-luc anti-miR-27b cells. Scale bar, 100 μm. (e) Flow cytometric analyses of the SP fraction of MCF7-luc derivatives in the presence and absence of Ko143. (f) Quantification of the SP fraction of MCF7-luc derivatives. The SP fraction was determined as the difference between the level of Hoechst 33342 staining in the presence and absence of Ko143. Data are represented as the mean±s.d. of n=3 replicates. Statistical significance was determined by Student’s t-test.