Figure 8: Metformin induces miR-27b-mediated suppression of ENPP1.
From: Loss of microRNA-27b contributes to breast cancer stem cell generation by activating ENPP1
(a,b) Immunoblot analyses of ENPP1 expression in MCF7-luc anti-miR-27b-DR (a) and ZR75-1-luc anti-miR-27b (b) cells incubated with metformin (0.1–10 mM) for 72 h. β-Actin was used as a loading control. (c) A schematic illustration of the miR-27b sensor construct used in the experiment shown in d. (d) Luciferase activity in MCF7 cells co-transfected with pTK-GLuc-27bs (50 ng) and pSV40-CLuc (50 ng), and then incubated with metformin for 48 h. The ratio of Gaussia to Cypridina luciferase activity (GLuc/CLuc) was determined. Data are represented as the mean±s.d. of n=3 replicates.
