Figure 7: Priming of Foxp3, Foxp3 expression and Treg in acetate-mediated suppression of AAD. | Nature Communications

Figure 7: Priming of Foxp3, Foxp3 expression and Treg in acetate-mediated suppression of AAD.

From: Evidence that asthma is a developmental origin disease influenced by maternal diet and bacterial metabolites

Figure 7

(a) Acetylation of histones at the Foxp3 promoter (adult=acetate from day -21 to end of model, offspring=acetate by mother D12-weaning). (b) Foxp3 expression in the lung. (c) Percentage and number of Treg cells in the lung. (d) Effect of acetate on Treg suppressive capacity (Treg isolated from the spleen of mice receiving water or acetate). The effect of anti-CD25 mediated Treg depletion on acetate mediated suppression of AAD in terms of (e) eosinophil cell number in BALF, (f) IL-5 and IL-13 release from MLN T cells, (g) serum IgE and (h) airway hyperresponsiveness in terms of airway resistance (RL). (i) AAD and the effect of anti-CD25 mediated Treg depletion in 3-week-old female offspring from mothers (C57Bl6) fed acetate, in terms of eosinophil cell number in BALF. Data represent mean+s.e.m., n=8. Significance is represented by *P<0.05, **P<0.01, ***P<0.001, Student’s t-test. One representative experiment of three is shown.

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