Figure 2: Silencing ZNF304 inhibits migration and proliferation.

The effect of ZNF304 silencing on invasion % (a), migration % (b) of HeyA8 cells and migration % of SKOV3IP1 cells (c). Migration and invasion percentages in ZNF304 siRNA-treated samples were calculated after normalization with control siRNA-treated samples. Box-and-whisker plots showing median (horizontal line), interquartile range (box) and maximum/minimum range (whiskers). All images shown are representative of three independent experiments and data are presented as mean±sem of n>3 experimental groups. *P≥0.05,**P≥0.01 and ***P≥0.001 (Student’s t-test). (d) Western blot analysis of ZNF304, p-Src (Y416) and total src levels 72 h post transfection with all ZNF304 siRNA sequences in HeyA8 cells. (e) Levels of focal adhesion kinase phosphorylation (Y397) after ZNF304 downregulation by all ZNF304 siRNA sequences in HeyA8 cells. (f) Immunoblotting results of β1 integrin and p-paxillin (Y31) and p-paxillin (Y118) after 72 h of ZNF304 siRNA treatment in HeyA8 cells. Cell-cycle arrest analysis of HeyA8 cells (g), SKOV3IP1 cells (h), A2780PAR cells (i) and A2780P20 cells (j) after 72 h transfection with ZNF304 siRNA. Cells were harvested at 72 h and were fixed, stained with propidium iodide and analysed by fluorescence-activated cell sorting. Data are presented as the percentage of cells as mean±s.e.m.of n≥3 experimental groups. *P≥0.05, **P≥0.01,***P≥0.001 (Student’s t-test).