Figure 4: Sample preparation by focused ion beam (FIB) for mass spectroscopy analyses of NHMUK R4493, NHMUK R12562 and fixed emu blood.
From: Fibres and cellular structures preserved in 75-million–year-old dinosaur specimens

(a) NHMUK R4493 with sampled location. (b) SEM of FIB sample preparation sequence: I, sample surface; II, platinum protecting layer; III, trench milling; IV, sample on copper grid holder ready for mass spectra acquisition. (c) NHMUK R12562 with sampling location. Scale bar, 5 μm. (d) Sample on grid holder before mass spectra acquisition. Scale bar, 5 μm. (e) SEM image of fixed emu blood with sampling location. (f) Sample on grid holder before mass spectra acquisition. Scale bar, 5 μm. The mass spectrum was obtained from the fresh surface in IV (d and f).