Figure 4: Knocking down the OsIAA11 expression partially rescues the lrt2 mutant phenotype. | Nature Communications

Figure 4: Knocking down the OsIAA11 expression partially rescues the lrt2 mutant phenotype.

From: Peptidyl-prolyl isomerization targets rice Aux/IAAs for proteasomal degradation during auxin signalling

Figure 4

(a) Analysis of the expression level of OsIAA11 in RNAi-transgenic seedlings (line numbers are given below the graph) by qRT–PCR. Error bars indicate s.d.; **P<0.01 (Student’s t-test). (b) Immunoblotting analysis of the accumulation of OsIAA11 protein in RNAi-transgenic seedlings (line numbers are given below the graph). Equal loading is verified by the analysis of HSP82 protein. Quantitative analysis of the relative level of OsIAA11 protein is presented below the blots. Error bars indicate s.d.; different letters indicate P<0.05 (LSD multiple range tests). (c–f) The root phenotype of 10-day-old seedlings with the indicated genotypes. In each panel, the right side shows the enlarged view of the boxed region at the left side. Scale bars, 1 cm. (g) Quantitative analysis of the percentage of lateral rooted plants in 10-day-old seedlings with the indicated genotypes (n≥30 for each genotype). Note that, knocking down the OsIAA11 expression in the wild-type background has no detectable effect on lateral root development.

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