Figure 6: DJ-1 deficiency confers metabolic protection in dietary and genetic models of obesity.
(a) Oxygen consumption (VO2) measured by indirect calorimetry, and (b) physical activity in chow- or HFD-fed female mice at 5 months of age (n=6 per group for chow-fed mice; n=13 per group for HFD-fed mice). HFD feeding was started at 2 months of age. (c) Relative weight of inguinal, perigonadal and interscapular brown (BAT) fat pads in HFD-fed female mice (n=11 for control and 8 for DJ-1 KO). (d) Rectal body temperature in HFD-fed female mice (n=6 for control and 4 for DJ-1 KO). (e) Food intake in HFD-fed mice, and (f) respiratory exchange ratio (RER) (n=6 per group for chow-fed mice; n=13 per group for HFD-fed mice). (g) Body weight in female mice (n=6 per group for chow-fed mice; n=12 per group for HFD-fed mice). (h) Haematoxylin and eosin staining of perigonadal fat sections from HFD-fed female mice and quantification of adipocyte size (n=5 per group). Scale bar, 80 μm. (i) Glucose tolerance test (GTT) (1 g kg−1; n=10 per group), (j) Insulin tolerance test (ITT) (1.5 U kg−1; n=12 per group) and (k) fasting serum insulin levels (n=9 per group) in HFD-fed female mice. Results are presented as mean±s.e.m. according to the two-tailed unpaired Student’s t-test. *P<0.05; **P<0.01; ***P<0.001.
