Figure 7: Attenuation of metabolic protection by NAC.
Two-month-old female DJ-1 KO mice and littermate controls were fed a HFD for 3 months and administered NAC in drinking water for 7 days (n=6 for control and 4 for DJ-1 KO). (a) H2O2 levels measured using the Amplex Red reagent in quadriceps homogenates or serum with and without NAC treatment. (b) ITT (1.5 U kg−1) and the corresponding area under the curve. (c) Immunoblot analysis of phospho-AMPKα(Thr172) protein levels in quadriceps lysates from NAC-treated mice. Full scan images of immunoblots are shown in Supplementary Fig. 9i. Results are presented as mean±s.e.m. according to the two-tailed unpaired Student’s t-test. *P<0.05; **P<0.01; ***P<0.001.
