Figure 2: Exclusion of nuclear contents in beaded apoptopodia. | Nature Communications

Figure 2: Exclusion of nuclear contents in beaded apoptopodia.

From: A novel mechanism of generating extracellular vesicles during apoptosis via a beads-on-a-string membrane structure

Figure 2

Localization of surface CD14 (a) and non-targeted intracellular GFP (b) in viable and apoptotic THP-1 monocytic cells. (c) Localization of Hoechst 33342, LysoTracker Red and MitoTracker Green staining in viable and apoptotic THP-1 cells. (d) Venn diagram of differentially abundant proteins in whole apoptotic sample and apoptotic body-enriched sample (a list of proteins identified in the proteomic analysis is shown in Supplementary Data 1). A total of 562 proteins were of low abundance in apoptotic body-enriched sample compared with whole apoptotic sample. Magenta and turquoise arrows indicate proteins that are in low or high abundance in apoptotic body-enriched sample compared with whole apoptotic sample, respectively. (e) Subcellular components depleted in apoptotic body-enriched sample compared with whole apoptotic sample are depicted based on FunRich analysis software. (f) Levels of histone H3, HMGB1 and β-actin in whole apoptotic sample and apoptotic body-enriched sample. (a–c,f) Data are representative of at least three independent experiments.

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