Figure 2: Expression of ACD6 and COR78. | Nature Communications

Figure 2: Expression of ACD6 and COR78.

From: Natural variation in timing of stress-responsive gene expression predicts heterosis in intraspecific hybrids of Arabidopsis

Figure 2

ACD6 and COR78 were subjected to diurnal regulation in two ecotypes, Col and C24, and downregulation in hybrids (arrows). (a,b) Relative expression levels (R.E.L.) of ACD6 (a) and COR78 (b) every 3 h in a 48-h period (ZT 0=dawn; mean±s.d., values were averaged from three biological replicates). Single and double asterisks indicate statistical significance levels at P<0.05 and P<0.01 using two-tailed Student’s t-test (compared with MPV), respectively. (c,d) Bioluminescent activities (counts per second in 1,000) of transgenic (T2) seedlings that expressed ACD6(Col):LUC in Col (green) or ACD6(C24):LUC in C24 (purple; c) and ACD6(C24):LUC in Col (red) or ACD6(Col):LUC in C24 (blue; d). (e,f) Bioluminescent activities of transgenic seedlings that expressed COR78(Col):LUC in Col (green) or COR78(C24):LUC in C24 (purple; e) and COR78(Col):LUC in C24 (blue) or COR78(C24):LUC in Col (red; f). Diurnal cycles of light (open) and dark (filled) and one of three replicate experiments (mean±s.e.m., n=16 individuals per transgenic line, three independent transgenic lines were examined) are shown.

Back to article page