Figure 5: Effects of knockdown or overexpression of abiotic and biotic genes on biomass. | Nature Communications

Figure 5: Effects of knockdown or overexpression of abiotic and biotic genes on biomass.

From: Natural variation in timing of stress-responsive gene expression predicts heterosis in intraspecific hybrids of Arabidopsis

Figure 5

(a) Rosettes of 3-week-old plants in F1(C24 × Ler) and its parents C24 and Ler. (b) Rosettes of 3-week-old plants in F1(C24 × Ws) and its parents C24 and Ws. Scale bars, 1 cm for all images. (c–f) Biomass of rosettes from 3-week-old seedlings in high-vigour crosses C24XLer (c) and C24XWs (d) and low-vigour crosses ColXLer (e) and ColXWs (f). Values were averaged from three biological replicates (±s.d.). (g) Biomass of Col and acd6-1 hyperactive mutant. (h,i) Dry weight (mg) of transgenic amiRACD6 lines in Col (h) and in C24 (i). (j,k) Dry weight (mg) of transgenic amiRCOR78 lines in Col (j) and in C24 (k). (l,m) Dry weight (mg) of transgenic 35 S:COR78 lines in Col (l) and in C24 (m). (n–p) Relative expression levels of COR78 expression in overexpression lines (n) and in artificial microRNA (amiRNA) lines (o) and of ACD6 in amiRNA lines (p). Values were averaged from three biological replicates (±s.d.). Single and double asterisks indicate statistical significance levels at P<0.05 and P<0.01 using two-tailed Student’s t-test, respectively, compared with the transgenic control (vector).

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