Figure 5: IL-23 induces expression of IL-1R on the surface of γδ T cells, whereas IL-1Ra suppresses its expression. | Nature Communications

Figure 5: IL-23 induces expression of IL-1R on the surface of γδ T cells, whereas IL-1Ra suppresses its expression.

From: IL-1 receptor antagonist-deficient mice develop autoimmune arthritis due to intrinsic activation of IL-17-producing CCR2+Vγ6+γδ T cells

Figure 5

(a,b) Concentrations of IL-17 in culture supernatants of magnetic-activated cell sorting (MACS)-purified (about 80%) (a) or FACS-purified (>99% pure) (b) splenic γδ T cells from pools of 16 WT mice (a,b) or 16 Il1a−/−b−/− mice (b) stimulated for 3 days with medium only, IL-1β, IL-23 or IL-23 plus IL-1β, without γδ TCR stimulation. IL-17 was detected by enzyme-linked immunosorbent assay (ELISA). ***P<0.001 versus WT mice (a); ***P<0.001 versus medium only (b) (unpaired Student’s t-test). (c–h) FACS-purified γδ T cells from pooled spleens of WT, Il1r1−/−, Il1a−/−b−/− or Il1rn−/− mice (11–16 mice each) were stimulated for 3 days with medium only, IL-1β, IL-23, IL-23 plus IL-1β or IL-23 plus IL-Ra. Flow cytometry of γδ T cells stained for surface IL-1R (c) and IL-23R (e) are shown. Quantification of IL-1R+ γδ T cells is indicated in d and g. Concentrations of IL-17 in culture supernatants were determined by ELISA (f,h). *P<0.05; **P<0.01; ***P<0.001 (versus WT mice) (unpaired Student’s t-test). Numbers in parentheses indicate the concentration of cytokines (ng ml−1). Representative data (c,e) and mean±s.e.m. (a,b,d,f,g,h) of triplicate cultures are shown. All data are representative of two or three independent experiments.

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