Figure 6: The Vγ6⁺ γδ T-cell subset is the major source of IL-17 in Il1rn^−/− mouse joints.

(a–c) GFP expression in joint-infiltrating γδ T cells from WT, Il1rn^−/− and Il17^g/gIl1rn^−/− mice at 16 weeks of age. Numbers refer to percentage in CD3ɛ⁺ γδ TCR⁺ cells (a, left). Quantification of GFP⁺ Vγ subsets in CD3ɛ⁺ γδTCR⁺ cells is shown (a, right). Numbers in the pie chart show the percentages of the indicated Vγ subset among GFP⁺ γδ TCR⁺ CD3ɛ⁺ cells, and represent the average of three mice (b). Mean fluorescence intensity of GFP in Vγ6⁺ (Vγ4⁻) or Vγ4⁺ cells is shown in c. *P<0.05, ***P<0.001 (versus Vγ4⁺ cells) (unpaired Student’s t-test). Data show the mean±s.e.m. of three mice. (d,e) Vγ (d) and Vδ (e) gene expression in γδ T cells. CD3ɛ⁺ γδ TCR⁺ (whole γδ T) cells from LNs (first row) or from joints (second row) of Il1rn^−/− mice and GFP⁺ CD3ɛ⁺ γδ TCR⁺ (GFP⁺ γδ T) (third row) or CD3ɛ⁺ γδ TCR⁻ (non-γδ T) (fourth row) cells from joints of Il17^g/gIl1rn^−/− mice, were sorted on a FACSAria, and Vγ and Vδ gene expression was analysed by RT–PCR.