Figure 3: Analysis of the PDGFR/PI3K/Akt pathway in NIH-3T3 fibroblasts by PDGF-BB stimulation.

(a) Cartoon illustrating signal transduction in PDGFR/PI3K/Akt pathway. (b) Dose–response plots of PDGFR Y857 2 min after stimulation, and (c) Akt S473 phosphorylation 5 min after stimulation. Data are expressed as fold changes relative to the response of cells exposed to a droplet containing only serum-free DMEM (that is, no stimulation). The ‘Average’ plots (left) are the average levels ±s.e.m. from all of the cells in three to four virtual microwells performed on different days. Representative ‘Histograms’ (middle) and single cell ‘Scatter plots’ (right) are shown with population mean±1 s.d. (representing a total of 383–1,288 cells per condition). Histogram distributions were offset vertically for comparison.