Figure 4: Single-cell Akt S473 phosphorylation in MDA-MB-231 cells.
From: Digital microfluidic immunocytochemistry in single cells
(a) Left: single-cell scatter plots of Akt activation after 0–7 min stimulation by 50 ng ml−1 of PDGF-BB. Data are shown as population mean±1 s.d. (representing a total of 946–2,219 cells per condition), with the percentage of hyperactive responders indicated above each time point. The most hyperactive responders (red box) were observed at 5 min stimulation. Right: differential single-cell scatter plots of Akt activation for the hyperactive and normal populations (population mean±1 s.d. representing 1,121 normal cells and 127 hyperactive cells) 5 min after stimulation. (b) Microarray scans of eight representative hyperactive cells observed 5 min after stimulation (red arrows indicate each cell) and table summarizing each cell’s Akt phosphorylation level, shape parameters and neighbour analysis. Scale bars, 25 μm.
