Figure 5: Post-translational degradation curves and ubiquitination levels of PRPS enzymes. | Nature Communications

Figure 5: Post-translational degradation curves and ubiquitination levels of PRPS enzymes.

From: Arginylation regulates purine nucleotide biosynthesis by enhancing the activity of phosphoribosyl pyrophosphate synthase

Figure 5

(a,b) Post-translational degradation of M- and R-PRPS1 and -PRPS2. In each set, the left panel shows a representative immunoblot and the right panel shows quantification of three independent repeats. Error bars represent s.e.m. The effectiveness of the cycloheximide treatment in these experiments was validated by measuring the degradation of the cyclin D1 on the same blot, as shown in Supplementary Fig. 3. (c) Ubiquitination levels of M- and R-PRPS1 and -PRPS2.

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