Figure 1: Cell-type-specific in situ labelling of proteins via FUNCAT.
From: Cell-selective labelling of proteomes in Drosophila melanogaster
(a–c) ANL incorporation into larval proteins is monitored via FUNCAT on targeted expression of dMetRSL262G-EGFP in neurons (a, elavC155-Gal4;;UAS-dMetRSL262G-EGFP), glia cells (b , repo-Gal4/UAS-dMetRSL262G-EGFP) and muscle cells (c, C57-Gal4/UAS-dMetRSL262G-EGFP) at larval neuromuscular junctions (muscles 6/7, segment A2). Co-staining with the neuron-specific marker anti-HRP (a–c) reveals that, wherever nerve terminal boutons (b), glial protrusions (g) and muscles (m) are in close contact, ANL-TAMRA signals are restricted to the dMetRSL262G-EGFP-expressing cell type (a–c). dMetRSL262G-EGFP is predominantly found in the cytosol, whereas TAMRA-harbouring proteins are detectable throughout cells including nuclei (n) and the bouton surrounding SSR area (c, dashed line). (d) Expression of dMetRSL262G-EGFP in a wing disc epithelium along the anterior–posterior border (ptc-Gal4;UAS-dMetRSL262G-EGFP) is accompanied by a respective confinement of ANL-Atto647N signals. The outline represents the shape of the entire disc. Scale bars, 10 μm (a,b); 5 μm (c); and 50 μm (d). SSR, subsynaptic reticulum.
