Figure 3: Neuronal ANL incorporation leaves NMJ morphology and prominent NMJ marker proteins unaffected.
From: Cell-selective labelling of proteomes in Drosophila melanogaster
Motor nerve terminals at NMJs from elavC155-Gal4;;UAS-dMetRSL262G-3xmyc L3 larvae fed without (a,c) or with (b,d) 4 mM ANL were visualized by the surface marker HRP and assessed for either FUNCAT-mediated TAMRA signals in combination with immunofluorescent labelling of the homophilic cell adhesion molecule FasII (a,b) or co-stained for the active zone marker Brp and PMCA to visualize the postsynaptic SSR compartment (c,d). There are no obvious differences between ANL- and non-ANL-reared animals. Scale bar, 5 μm. NMJ, neuromuscular junction; SSR, subsynaptic reticulum.
