Figure 4: NCAT monitoring of protein synthesis over time.
From: Cell-selective labelling of proteomes in Drosophila melanogaster
Newly synthesized proteins were monitored by FUNCAT in motor neurons of OK371-Gal4;UAS-dMetRSL262G-EGFP third instar larvae that were exposed to ANL for 12, 24 or 48 h (a). Selective dMetRSL262G-EGFP expression in motor neurons was confirmed by EGFP fluorescence (a, upper panel). Fluorescence intensity of TAMRA tagged newly synthesized proteins increased with increasing ANL exposure times (a, lower panel). Identical confocal settings were used for acquisition of all images, and representative images are shown. Scale bar, 20 μm. (b) Quantification of fluorescence intensities confirmed the positive correlation between FUNCAT labelling intensity and the duration of ANL exposure. Averages±s.d. relative to 24 h ANL exposure (100%) are shown. Mann–Whitney U-test, n=10–11, **P≤0.01, ***P≤0.001 12 versus 24 h: **P=0.001530673; 12 versus 48 h: ***P=1.08E−04; 24 versus 48 h: ***P=9.34E−05. s.d. (12 h ANL): 11.83; s.d. (24 h ANL): 59.28; s.d. (48 h ANL): 103.50.
